Introduction

Quantification | quantification & validation

Processing of kit data is performed in the Quantification module by following these steps.

1. Upload raw data: Drop MS data files from a kit run into WebIDQ
2. Integration | LC part: Define integration windows and perform peak integration
3. Calibration | LC part: External calibration of LC data
4. Integration | FIA/FIA XL part: Define integration windows and perform integration
5. Validation: Performance evaluation of a kit run

Quantification workflow guidance

The quantification workflow guidance shows the integration, calibration and validation status in the top right corner of the Quantification module. Three colored statuses are available: .

• White indicates a step has yet to be performed
• Red indicates a step must be performed again
• Blue indicates that a step is complete

Moving the mouse over the workflow guidance tool will display additional information:

1. Integration windows
   Define integration windows!
   Integration windows were defined.

2. Integration
   Integration required!
   Integration windows were altered. Integration required!
   Integration was performed.

3. Calibration (LC data only)
   Calibration required!
   Integration or calibration parameters were altered. Calibration required!
   Calibration was performed.

4. Validation
   Validation required!
   Integration or calibration parameters were altered. Validation required!
   Validation was performed.

Clicking on the squares will take you to the respective section: Integration, Calibration, and Validation